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They are biologically active and can trigger inflammatory and other cellular responses, even at low concentrations in susceptible biological systems.",{"type":113,"children":549},[550],{"type":116,"value":551},"In cell-based research, endotoxin contamination can influence experimental outcomes, particularly in sensitive applications involving immune cells, cytokine measurements and cell proliferation assays.",{"type":113,"children":553},[554],{"type":116,"value":117},{"type":113,"children":556},[557],{"type":116,"value":558},"This makes endotoxin control important for several reasons:",{"type":560,"style":561,"children":562},"list","bulleted",[563,574,583],{"type":564,"children":565},"listItem",[566],{"type":113,"children":567},[568,572],{"type":116,"marks":569,"value":571},[570],"strong","Cellular responses",{"type":116,"value":573},": Endotoxins may activate sensitive cell populations and alter their behaviour.",{"type":564,"children":575},[576],{"type":113,"children":577},[578,581],{"type":116,"marks":579,"value":580},[570],"Experimental reliability:",{"type":116,"value":582}," Uncontrolled endotoxin exposure can introduce variability and affect the interpretation of results.",{"type":564,"children":584},[585],{"type":113,"children":586},[587,590],{"type":116,"marks":588,"value":589},[570],"Material quality",{"type":116,"value":591},": Defined endotoxin specifications provide greater control over materials that come into direct contact with cells.",{"type":113,"children":593},[594],{"type":116,"value":537},{"type":113,"children":596},[597],{"type":116,"value":598},"For researchers working with endotoxin-sensitive cell populations and assays, defined endotoxin specifications are an important consideration when selecting materials that come into direct contact with cells.",{"id":600,"__typename":509,"content":601},"TeA-92nvQWeuspKghyY24g",{"value":602},{"schema":108,"document":603},{"type":110,"children":604},[605,609,613],{"type":539,"level":540,"children":606},[607],{"type":116,"value":608},"Quality considerations for cryopreservation media",{"type":113,"children":610},[611],{"type":116,"value":612},"Freezing and thawing expose cells to physical and osmotic stresses. Cryoprotectants help mitigate these stresses. Beyond cryoprotective performance, the composition and quality of the freezing medium should also be assessed because it comes into direct contact with cells.",{"type":113,"children":614},[615],{"type":116,"value":616},"Media, supplements and cryoprotective agents all become part of the cellular environment during processing. Selecting materials with appropriate quality specifications can help establish more controlled and consistent workflows.",{"__typename":618,"id":619,"reversed":297,"blueBackground":297,"sbsImage":620,"text":630},"SideBySideBlockRecord","D9E0SZ9MTHqPzjc8UYxGYw",{"image":621},[622],{"url":623,"urlBaseName":624,"thumbnailUrl":625,"type":138,"extension":139,"description":140,"alt":140,"fileId":626,"fileName":627,"folderId":442,"token":140,"external":140,"cf":628,"downloadTemplateId":145,"originalWidth":445,"originalHeight":446,"originalSize":629,"originalUrl":623},"https://pharmacosmos.kontainer.com/cdn/N91Rzlr90S12/careful-vial-review-in-quality-lab-1.png","https://pharmacosmos.kontainer.com/cdn/N91Rzlr90S12/careful-vial-review-in-quality-lab-1","https://pharmacosmos.kontainer.com/cdn/N91Rzlr90S12/thumbnail/fullscreen/careful-vial-review-in-quality-lab-1.webp",24571218,"Careful vial review in quality lab-1.png",[],1680368,{"content":631},{"value":632,"links":698},{"schema":108,"document":633},{"type":110,"children":634},[635,639,677,681,684,688,691,694],{"type":113,"children":636},[637],{"type":116,"value":638},"Alongside cryoprotective performance, important quality considerations include:\n\n",{"type":560,"style":561,"children":640},[641,650,659,668],{"type":564,"children":642},[643],{"type":113,"children":644},[645,648],{"type":116,"marks":646,"value":647},[570],"Endotoxin levels:",{"type":116,"value":649}," Defined specifications and analytical controls.",{"type":564,"children":651},[652],{"type":113,"children":653},[654,657],{"type":116,"marks":655,"value":656},[570],"Sterility:",{"type":116,"value":658}," Appropriate microbiological quality for the intended application.",{"type":564,"children":660},[661],{"type":113,"children":662},[663,666],{"type":116,"marks":664,"value":665},[570],"Composition and consistency:",{"type":116,"value":667}," Reliable formulation characteristics across batches.",{"type":564,"children":669},[670],{"type":113,"children":671},[672,675],{"type":116,"marks":673,"value":674},[570],"Manufacturing quality:",{"type":116,"value":676}," Controlled production processes and supporting documentation.",{"type":113,"children":678},[679],{"type":116,"value":680},"\n",{"type":113,"children":682},[683],{"type":116,"value":117},{"type":113,"children":685},[686],{"type":116,"value":687},"Endotoxin levels can be quantified using established analytical methods, including Limulus amebocyte lysate (LAL)-based assays. These measurements enable manufacturers to establish specifications and monitor endotoxin levels as part of their quality-control strategy.",{"type":113,"children":689},[690],{"type":116,"value":680},{"type":113,"children":692},[693],{"type":116,"value":117},{"type":113,"children":695},[696],{"type":116,"value":697},"The appropriate endotoxin limits ultimately depend on the intended application and the requirements of the finished product. Nevertheless, selecting materials with controlled endotoxin specifications helps reduce one potential source of variability in sensitive cell-processing workflows.",[],{"id":700,"__typename":509,"content":701},"Pugc1YQDQg-5i4KFcEs9vA",{"value":702},{"schema":108,"document":703},{"type":110,"children":704},[705,709,713,717,720,723,727,747,750,754,758,761,765,768,773],{"type":539,"level":540,"children":706},[707],{"type":116,"value":708},"PentaHibe: Cryopreservation with material quality in mind",{"type":113,"children":710},[711],{"type":116,"value":712},"PentaHibe offers a carbohydrate-based approach to cryopreservation, using pentaisomaltose as a non-penetrating, extracellular cryoprotectant.",{"type":113,"children":714},[715],{"type":116,"value":716},"Compared with conventional cryopreservation formulations that often contain around 10% DMSO, PentaHibe provides flexibility to reduce or eliminate DMSO, depending on the cell type, formulation and application.",{"type":113,"children":718},[719],{"type":116,"value":117},{"type":113,"children":721},[722],{"type":116,"value":117},{"type":113,"children":724},[725],{"type":116,"value":726},"The portfolio includes two options:",{"type":560,"style":561,"children":728},[729,738],{"type":564,"children":730},[731],{"type":113,"children":732},[733,736],{"type":116,"marks":734,"value":735},[570],"PentaHibe Complete – Ready-to-use, 2% DMSO: ",{"type":116,"value":737},"A ready-to-use cryopreservation formulation containing 10% pentaisomaltose and 2% DMSO, offering an alternative to conventional higher-DMSO formulations.",{"type":564,"children":739},[740],{"type":113,"children":741},[742,745],{"type":116,"marks":743,"value":744},[570],"PentaHibe Base – DMSO-free: ",{"type":116,"value":746},"A 40% pentaisomaltose concentrate designed for customised cryopreservation formulations, allowing researchers to develop DMSO-free or reduced-DMSO approaches according to their specific cell-processing requirements.",{"type":113,"children":748},[749],{"type":116,"value":680},{"type":113,"children":751},[752],{"type":116,"value":753},"Beyond cryoprotective functionality, PentaHibe is manufactured under cGMP conditions in Denmark, with defined quality specifications, including controlled endotoxin levels.",{"type":113,"children":755},[756],{"type":116,"value":757},"This combination of formulation flexibility, controlled manufacturing and pharmaceutical expertise supports researchers and developers seeking to consider both cryopreservation performance and material quality.",{"type":113,"children":759},[760],{"type":116,"value":537},{"type":113,"children":762},[763],{"type":116,"value":764},"When selecting a cryopreservation medium, both cryoprotective performance and product quality should be considered. These are distinct but complementary considerations; cryoprotective performance relates to preservation of cells through freezing and thawing, while defined quality specifications provide control over the material to which the cells are exposed. PentaHibe combines a carbohydrate-based cryopreservation approach with controlled pharmaceutical manufacturing and defined quality specifications.",{"type":113,"children":766},[767],{"type":116,"value":537},{"type":113,"children":769},[770],{"type":116,"marks":771,"value":772},[570],"Discover PentaHibe and explore a different approach to cryopreservation.",{"type":113,"children":774},[775],{"url":776,"meta":777,"type":175,"children":781},"https://pentahibe.com/products/",[778],{"id":779,"value":780},"target","_blank",[782,785],{"type":116,"marks":783,"value":784},[570],"CTA: ",{"type":116,"marks":786,"value":788},[570,787],"underline","Explore PentaHibe Products",1793702110407]