Uncompromising Cryopreservation

PentaHibe is a carbohydrate-based alternative to DMSO for cryopreservation
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A new golden standard in Cryopreservation

PentaiHibe® (containing Pentaisomaltose®) is a safe and effective alternative to dimethyl sulfoxide (DMSO) for cryopreservation of cells.

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What is PentaHibe?

PentaiHibe® (containing Pentaisomaltose®) is a safe and effective alternative to dimethyl sulfoxide (DMSO) for cryopreservation of cells.

Cryoprotecting agents are used to protect biological tissue from freezing-related damage during cryopreservation. Today, dimethyl sulfoxide – also known as DMSO – is the standard cryoprotective agent in view of its proven efficacy. However, DMSO can be toxic to tissues and cells and can lead to adverse reactions and side effects including nausea, vomiting, abdominal cramps and headache.

Furthermore, DMSO has a strong and unpleasent smell that can be a nuisance for health care professionals, patients, and their families1.

That is why, Pharmacosmos has developed PentaHibe® as a safe and effective DMSO free alternative. PentaHibe® is a proprietary formulation of isomalto-oligosaccharides conforming to the European Pharmacopeia (EP) and U.S. Pharmacopeia (USP) for dextran 1.

PentaHibe® has a very low toxicity and offers a wide range of benefits for both patients and professionals compared to existing solutions.

PentaHibe® is optimized for cryopreservation of hematopoietic stem cells, mesenchymal stem cells and T-cells and will help professionals offer better treatment resulting in improved safety for patients all over the world.

Key Benefits of PentaHibe

  • Efficacy – Cryopreservation of Hematopoietic Stem Cells (HSCs) without DMSO

    Cryopreservation of HSCs was compared in media with PentaHibe® (16% Pentaisomaltose®) and a media with 10% DMSO used in clinical practice2

    Relative to the media with 10% DMSO, PentaHibe® demonstrated

    • On par viability and recovery as well as the same colony-forming cell potential of HSCs2
    • Effective engraftment in an animal model (formation of myeloid and lymphoid cells)2
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Pharmacosmos offers PentaHibe®to companies that have interest in using PentaHibe® for commercial purposes in the cell therapy industry. 


  1. Svalgaard J. et al "Pentaisomaltose®, an alternative to DMSO, Engraftment of Cryopreserved HumanDC34+ Cells in immunodeficient Mice" Cell Transplantation 2018, Vol 27(9) 1407-1412
  2. Shu Z. et al "Hemapotoitic Stem Cell Transplantation with Cryopreserverd Grafts: Adverse Reactions after Transplantation and CryoProtectant Removal Prior to Infusion", Bone Marrow Transplant 2014 April; 49(4) 469-476
  3. Svalgaard J, et al "Cryopreservation of adipose-derived stromal/stem cells using 1-2% ME2SO (DMSO) in combination with pentaisomaltose®: And effective and less toxic alternative to comparable freezing media" , Cryobiology 22 June 2020.
  4. Haastrup EK. et. Al, “DMSO (Me2SO) concentrations of 1-2% in combination with pentaisomaltose are effective for cryopreservation of T cells”, Transfusion and Apheresis Science, 2021 
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    Pharmacosmos is the only dextran manufacturer holding certificates from the US FDA and European Directorate for the Quality of Medicines (EDQM)

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    Quality Delivered

    Pharmacosmos delivers a unique dextran quality through a production technology that completely avoids the use of organic solvents and a quality system that meets the strictest cGMP standards for human medicines

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    Global Service

    Pharmacosmos sells and ships directly to clients everywhere in the World. We deliver Pharmaceutical Quality Dextran of the highest standards, including those of the European Pharmacopoeia (EP), the United States Pharmacopoeia (USP), and the Japanese Pharmacopoeia (JP).