PentaHibe® is a carbohydrate-based cryoprotective agent, offering an efficacious and more safe alternative to DMSO for cryopreservation of cells.

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What is PentaHibe?

PentaHibe® (containing Pentaisomaltose®) is a safe and effective alternative to dimethyl sulfoxide (DMSO) for cryopreservation of cells.

Cryoprotecting agents are used to protect biological tissue from freezing-related damage during cryopreservation. Today, DMSO is the standard cryoprotective agent in view of its proven efficacy. However, DMSO can be toxic to tissues and cells and can lead to adverse reactions and side effects including nausea, vomiting, abdominal cramps and headache.

Furthermore, DMSO has a strong and unpleasent smell that can be a nuisance for health care professionals, patients, and their families1.

That is why Pharmacosmos has developed PentaHibe® as a safe and effective cryoprotective agent, allowing you to reduce or even do without DMSO, without compromising on efficacy. PentaHibe® is a proprietary formulation of isomalto-oligosaccharides conforming to the European Pharmacopeia (EP) and U.S. Pharmacopeia (USP) for dextran 1.

PentaHibe® is optimized for cryopreservation of hematopoietic stem cells, mesenchymal stem cells and T-cells and will help professionals offer better treatment resulting in improved safety for patients all over the world.

Key Benefits of PentaHibe®

Proven efficacy

  • 16% PentaHibe® has comparable effect to 10% DMSO when assessing hematopoietic stem cells (HSCs)1
  • 10% PentaHibe® with 2% DMSO has shown superior effect to other commercial media with 2% DMSO and 10% DMSO when assessing mesenchymal stem cells (MSCs)2
  • 10% PentaHibe® with 2% DMSO is superior on viability to DMSO 10% as single component in the cryopreservation of T cells3

Safe and non-toxic

  • PentaHibe® is a non-toxic carbohydrate, free of animal components
  • PentaHibe® reduces the need for DMSO, and therefore the risk of DMSO-related side effects such as odor, nausea, and vomiting

Environmentally friendly

  • The production of PentaHibe® is entirely water-based, with no use of organic solvents. PentaHibe® is metabolized to glucose and water

Explore the data

Explore the publications on PentaHibe, showcasing its effect on cryopreservation of different cell types. 

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References 

  1. Svalgaard J. et al. ”Pentaisomaltose, an Alternative to DMSO. Engraftment of Cryopreserved Human CD34+ Cells in Immunodeficient Mice”, Cell Transplantation 2018, Vol. 27(9) 1407-1412
  2. Svalgaard J. et al. “Cryopreservation of adipose-derived stromal/stem cells using 1-2% ME2SO (DMSO) in combination with pentaisomaltose: An effective and less toxic alternative to comparable freezing media”, Cryobiology 22 June 2020
  3. Haastrup EK. et al. “DMSO (Me2SO) concentrations of 1-2% in combination with pentaisomaltose are effective for cryopreservation of T cells”, Transfusion and Apheresis Science, 2021
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    Approved

    Pharmacosmos is the only dextran manufacturer holding certificates from the US FDA and European Directorate for the Quality of Medicines (EDQM)

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    Quality Delivered

    Pharmacosmos delivers a unique dextran quality through a production technology that completely avoids the use of organic solvents and a quality system that meets the strictest cGMP standards for human medicines

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    Global Service

    Pharmacosmos sells and ships directly to clients everywhere in the World. We deliver Pharmaceutical Quality Dextran of the highest standards, including those of the European Pharmacopoeia (EP), the United States Pharmacopoeia (USP), and the Japanese Pharmacopoeia (JP).