PentaHibeComplete

Objectives: to compare and evaluate the viability and recovery of T cells purified from buffy coats after cryopreservation with a self-made 10% DMSO formulation, a commercial product with 10% DMSO, or ready-to-use PentaHibe Complete.  

Results: T cells purified from buffy coats from healthy donors and cryopreserved with PentaHibe Complete show similar viability and recovery to those cryopreserved with self-made and commercial formulas containing 10% DMSO.4

Objectives: to compare and evaluate the proliferation of T cells purified from buffy coats after cryopreservation with a self-made 10% DMSO formulation, a commercial product with 10% DMSO, and ready-to-use PentaHibe Complete.  

Results: T cells purified from buffy coats and cryopreserved with PentaHibe Complete show similar proliferation as those cryopreserved with self-made and commercial formulas containing 10% DMSO.4

Objectives: to evaluate the expansion capabilities of T cells post-cryopreservation using PentaHibe Complete compared to a commercial product containing 10% DMSO. T cells were stimulated with IL-2 and anti-CD3/CD28 antibody beads, and their expansion was monitored over a 15-day period. Expansions were calculated from the accumulated count of viable T cells on selected days after thawing.  

Results: T cells purified from buffy coats of healthy donors and cryopreserved with PentaHibe Complete showed similar expansion comparable to those preserved in a commercial formulation containing 10% DMSO.6

Objectives: To evaluate the post-thaw viability of T cells cryopreserved with PentaHibe Complete. Viability was measured at different time points (0, 6, and 24 hours post-thaw). The study aimed to assess the effectiveness of PentaHibe® Complete in maintaining high viability for downstream applications in cell therapy research.  

Results: PentaHibe Complete demonstrated exceptional post-thaw viability, with >90% viability immediately after thawing. Over a 24-hour culture period, viability remained high (80-85%), providing an extended window for handling and processing. These findings confirm that PentaHibe Complete is an effective cryoprotectant for preserving the integrity and functionality of T cells in translational research.6

Each data point represents the mean viability of T cells purified from three donor buffy coats, with each donor analysed in triplicate. Cells were frozen using a controlled-rate freezer (4°C, -1°C/min to 0°C, -2°C/min to -45°C, -5°C/min to -100°C) and transferred to a cryogenic freezer. After thawing, the cells were cultured in RPMI medium.

Objectives: To evaluate the preservation of post-thaw T cell functionality and effector response following cryopreservation using PentaHibe Complete compared with a commercial cryopreservation product (Product C). Primary human T cells were cryopreserved using a controlled-rate freezing protocol, stimulated immediately after thaw with PMA/ionomycin, and functional activity was assessed by measuring interferon-gamma (IFN-γ) secretion. Values represent mean ± SD (n = 9; 3 donors with triplicate measurements). 

Results: T cells cryopreserved in PentaHibe Complete demonstrated strong IFN-γ secretion immediately after thaw, comparable to Product C. These findings show that PentaHibe Complete effectively maintains T cell effector responses following cryopreservation, making it well suited for translational and cell therapy applications.6

Objectives: to compare and evaluate the viability of adipose tissue derived human mesenchymal stem cells after cryopreservation with a self-made 10% DMSO formulation, a commercial product with 10% DMSO, or ready-to-use PentaHibe Complete.  

Results: PentaHibe Complete is suitable for the cryopreservation of adipose tissue derived human mesenchymal stem cells.5

Objectives: to compare and evaluate the viability and recovery of CHO cells after cryopreservation with a self-made 10% DMSO formulation, a commercial product with 10% DMSO, or ready-to-use PentaHibe Complete.

Results: CHO cells cryopreserved with PentaHibe Complete show similar viability and recovery to those cryopreserved with self-made and commercial formulas containing 10% DMSO.6

Objectives: to compare and evaluate the viability and recovery of HEK293 cells after cryopreservation with Pentahibe Complete or with a commercial product with 10% DMSO.

Results: HEK cells cryopreserved with PentaHibe Complete show similar viability and recovery to those cryopreserved with commercial formula containing 10% DMSO.6

Objectives: to evaluate the ability to successfully establish organoid cultures from intestinal mucosal biopsies (duodenum, ileum, and colon) cryopreserved with either Pentahibe Complete or a conventional 10% DMSO-based medium.

Results: biopsies cryopreserved with Pentahibe Complete demonstrated a 100% organoid establishment success rate, compared to 64.29% for the standard freezing medium, across all intestinal segments.7

Objectives: To assess the impact of Pentahibe Complete versus a conventional 10% DMSO-based freezing medium on both the number and area expansion of organoids derived from duodenum (Duo) biopsies over time, using time-lapse imaging analysis.

Results: Biopsies cryopreserved in Pentahibe Complete consistently produced a higher number of organoids and exhibited substantially greater area expansion compared to those frozen in a standard 10% DMSO-based medium over a 180-hour observation period. Time-lapse imaging confirmed these findings, revealing increased organoid count (Fig. 1a) and larger organoid area (Fig. 1b) over time, with representative images demonstrating robust growth by day 13 post-thaw (Fig. 1c). These results underscore Pentahibe Complete as an advanced solution for preserving tissue integrity and promoting efficient organoid generation.⁷